Insertion junction: LMJ.RY0402.141667_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.141667
Chromosome:chromosome 1
Location:5377520
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g037800 TRL7,TRX21 (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGGTATACCCACAGACCCTGCTGATGA
Internal bar code:TTAATGGGTACTAACACCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:605
LEAP-Seq percent confirming:99.5256
LEAP-Seq n confirming:3357
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAGAGCGACATGATTGA
Suggested primer 2:GTGTGGGTGAGGACATGTTG