Insertion junction: LMJ.RY0402.141667_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.141667
Chromosome:chromosome 1
Location:5378104
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g037800 TRL7,TRX21 (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGCCGCTCCGCCAGCACCGACGTCTCAA
Internal bar code:GACGCGGTCGACCTCTATTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:318
LEAP-Seq percent confirming:99.6608
LEAP-Seq n confirming:1469
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATACTCACGTGCTCACCCC
Suggested primer 2:ACTCGTGTCGTCATCAGCAG