Insertion junction: LMJ.RY0402.142008_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.142008
Chromosome:chromosome_9
Location:3832786
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g391801 VSP4 Hydroxyproline-rich glycoprotein, cell wall protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ATTCCTTGAACAGTGAACTTCATCTGTGCT
Internal bar code:GTGCACCCCCCGGCTTGTATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:438
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTTGCTATTTCCCGTT
Suggested primer 2:GTGCATGTTGGTTGATACGC