| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.142112 |
| Chromosome: | chromosome 5 |
| Location: | 446434 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232550 | GPM4,PGM4,CA1P1 | (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACGCCCGACGTCAACTCCTACCTCGTC |
| Internal bar code: | TCGGGCTCGTTCGTAATAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 98.9796 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACACTGCTGCTTCATTA |
| Suggested primer 2: | ACCACGTGTACGGTGTTGAA |