Insertion junction: LMJ.RY0402.142230_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.142230
Chromosome:chromosome 17
Location:3778984
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g727600 (1 of 1) PF06650//PF12624//PF16908//PF16909 - SHR-binding domain of vacuolar-sorting associated protein 13 (SHR-BD) // N-terminal region of Chorein or VPS13 (Chorein_N) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Vacuolar-sorting-associated 13 protein C-terminal (VPS13_C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGGACCCAGAAAGCAGCATTCCTGGCA
Internal bar code:TGCCTGAACTGCAGGAACTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:85.8552
LEAP-Seq n confirming:5833
LEAP-Seq n nonconfirming:961
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTGGTACAGGCTATGC
Suggested primer 2:CCGCACTTCCTTCTCTTACG