| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.142637 |
| Chromosome: | chromosome 16 |
| Location: | 863143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647950 | (1 of 33) IPR015797 - NUDIX hydrolase domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCGGGTGGGAGGCTAGGTCGGCGGA |
| Internal bar code: | GTGGAAGTCGGGCGTGGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 93.2692 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCGTGCTATGTTGTTGG |
| Suggested primer 2: | AAATCCCCGAGAGCAAAAAC |