| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143057 |
| Chromosome: | chromosome_12 |
| Location: | 7635712 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g554850 | TRX7,TRXh1,TRXh2 | Thioredoxin h2, cytosolic | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAGCCCGCGTCGTAGGCAATTTAGGCAAC |
| Internal bar code: | GATAGCGCGGAGCTGTATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 95.7739 |
| LEAP-Seq n confirming: | 3422 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCCCAACATCACATTCC |
| Suggested primer 2: | GAGTGGAGATGGTGTTCGGT |