| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143186 |
| Chromosome: | chromosome 16 |
| Location: | 2729661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662600 | BST1,LCI11,LCI11C | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); Bestrophin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAAATTGCTTATGACTCGGTTGATGTTC |
| Internal bar code: | TTTGCTCCGGCTCGTCCGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.9235 |
| LEAP-Seq n confirming: | 1307 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCCGACGAGAGCGTAAT |
| Suggested primer 2: | ATGGGACAGTGGTAAGGCAG |