| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143358 |
| Chromosome: | chromosome 1 |
| Location: | 5440277 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038151 | (1 of 1) PTHR23069:SF0 - TAT-BINDING HOMOLOG 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGGTCGACCGGAATCGGCGTCCGACT |
| Internal bar code: | GCGGAGGTACACAATCGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 99.359 |
| LEAP-Seq n confirming: | 310 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCGAGCTCTTTGTTG |
| Suggested primer 2: | GCAACCCTTCTCTATGCTGC |