| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143446 |
| Chromosome: | chromosome 3 |
| Location: | 1885964 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155001 | ISA1,STA7 | (1 of 1) PTHR10357:SF169 - ISOAMYLASE 1, CHLOROPLASTIC; Isoamylase, starch debranching enzyme 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCACAAAAGTTGGTTGCCCGCCAGTCGG |
| Internal bar code: | TGGTGGTGTAGGAGGAGAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 1812 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGGCGCTAGTCAACAA |
| Suggested primer 2: | CATGACTACCAACACACCCG |