| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143543 |
| Chromosome: | chromosome 16 |
| Location: | 3814599 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689650 | ATG8,APG8 | Autophagy-related protein; (1 of 1) K08341 - GABA(A) receptor-associated protein (GABARAP, ATG8, LC3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATTATCAGATTTAAGTGCTGCCGGACC |
| Internal bar code: | TTCTCGCTTCTTTTTACCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 96.9811 |
| LEAP-Seq n confirming: | 1028 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTGTACGTCATCCGCA |
| Suggested primer 2: | GGAAGGCTAGATGCACGAAG |