| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143715 |
| Chromosome: | chromosome 12 |
| Location: | 9252140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGGGCCGGGCTGCAGCAGCGGTGGCGC |
| Internal bar code: | GCCACAACTGTGCGTGCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 99.4453 |
| LEAP-Seq n confirming: | 3944 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTTGAGCGAGGAGGAG |
| Suggested primer 2: | TGACCCACTACTGAGGAGCC |