| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143737 |
| Chromosome: | chromosome 12 |
| Location: | 9460549 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539650 | (1 of 1) PF00530//PF08016 - Scavenger receptor cysteine-rich domain (SRCR) // Polycystin cation channel (PKD_channel) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCGAGCGAGTGCTACAGTAATTGTGTG |
| Internal bar code: | GCTACTCGGACAACTGTTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 85.8044 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTACATGAAGCCCCAA |
| Suggested primer 2: | ACACACACACACACACACCG |