| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143909 |
| Chromosome: | chromosome 9 |
| Location: | 4035773 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393136 | (1 of 5) PF01417 - ENTH domain (ENTH) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCTTGAACTTTTGTGTACATACAATAC |
| Internal bar code: | TATGTCTTAGCAACTATTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 97.8441 |
| LEAP-Seq n confirming: | 3903 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGACGTAGTTGACCGA |
| Suggested primer 2: | GCCCAAGACTATCCCGTGTA |