| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143997 |
| Chromosome: | chromosome_3 |
| Location: | 3470519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g167550 | POC3,CEP290 | Centriole proteome protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGAAGATGGTGGGGGGGGGCAGAGTGGGG |
| Internal bar code: | GCGGGGCTCGAGATACGGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 98.1919 |
| LEAP-Seq n confirming: | 5485 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATTACGAGCAGCAGGTG |
| Suggested primer 2: | TGTTCTTCTCCAGCACGTTG |