Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.144402 |
Chromosome: | chromosome_4 |
Location: | 2956420 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g224800 | VAMP74,VAM4 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTGCCTCCACTTTGTCCAGGAAGGCGGAG |
Internal bar code: | CAGCTTATGACGGGAACCCCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 918 |
LEAP-Seq percent confirming: | 99.7217 |
LEAP-Seq n confirming: | 2150 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCACCACGATCTTCATCTT |
Suggested primer 2: | GCAAACATGCAACCAAACAC |