| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144472 |
| Chromosome: | chromosome 9 |
| Location: | 3283251 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388097 | TOC80,OEP80 | Putative outer membrane chloroplast protein; (1 of 2) K07277 - outer membrane protein insertion porin family (SAM50, TOB55, bamA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGGTCTTTACGGAAGCCAGGCGTCAC |
| Internal bar code: | GCCAAGGCGGGAACGGTTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.8519 |
| LEAP-Seq n confirming: | 2697 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGAGTCTTTCGCCAGTTT |
| Suggested primer 2: | CTTCTTCGACTTCGGGTCTG |