| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144499 |
| Chromosome: | chromosome 16 |
| Location: | 1494485 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652950 | (1 of 1) K11274 - chromosome transmission fidelity protein 4 (WDHD1, CTF4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAAACGCCTTGAGCAGCGCCGCGTCG |
| Internal bar code: | ATCCGCTGCGTGCGGTCTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 94.5437 |
| LEAP-Seq n confirming: | 3916 |
| LEAP-Seq n nonconfirming: | 226 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTCTCGACCAGTGCGT |
| Suggested primer 2: | AGAGATGCGCACTGACACAC |