Insertion junction: LMJ.RY0402.144552_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144552
Chromosome:chromosome 8
Location:601776
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g359650 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTCACCGTTTTCGGATCTTGGGTGTT
Internal bar code:GTCGTTCCCACACCCCGTTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:151
LEAP-Seq percent confirming:99.8842
LEAP-Seq n confirming:2587
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCTCGTGCCTCTTCAAC
Suggested primer 2:TGCAGTCGAGAAATGCAAAC