| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145008 |
| Chromosome: | chromosome 13 |
| Location: | 4546656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603700 | DII4,NSG4,ACT1,IDA5 | Actin, flagellar inner arm dynein subunit; (1 of 1) K10355 - actin, other eukaryote (ACTF) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGTGCTACGATGCTGCAACAGAAGC |
| Internal bar code: | CCAGGTAAGGTGTCATTAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1314 |
| LEAP-Seq percent confirming: | 99.6684 |
| LEAP-Seq n confirming: | 17133 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCACGAGAGAAGGCAT |
| Suggested primer 2: | CACAATACCGTGCTCAATGG |