Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.145937 |
Chromosome: | chromosome_12 |
Location: | 6549413 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g539100 | HEL57 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGCCATCGTCAAGTCGCTGTACGACGAGC |
Internal bar code: | CTATCGGCCAATCAGTATAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 199 |
LEAP-Seq percent confirming: | 95.7286 |
LEAP-Seq n confirming: | 381 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATGCGGATGTTACAGAGGG |
Suggested primer 2: | GATGGTCAGTCTGATGGCCT |