| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145938 |
| Chromosome: | chromosome 3 |
| Location: | 6314025 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193400 | (1 of 2) PTHR30603 - RNA POLYMERASE SIGMA FACTOR RPO | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACGGGAGGGACCATTGTCGTGCAAGCT |
| Internal bar code: | CGCAGGGGCACCTGGCAGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 90.5906 |
| LEAP-Seq n confirming: | 1810 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTGAGTACGACCTTCC |
| Suggested primer 2: | GCAGCTACAACGGAAAGGAG |