| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.146151 |
| Chromosome: | chromosome 11 |
| Location: | 3625939 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482150 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGATGGATACGGTGCGTGCCGGTAGCG |
| Internal bar code: | CAGGGGCTTCCATTGCATTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 98.843 |
| LEAP-Seq n confirming: | 2990 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGGCGGTTATGGTGGTT |
| Suggested primer 2: | AATGCGGATGTTACAGAGGG |