| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.146784 |
| Chromosome: | chromosome 13 |
| Location: | 3552730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588150 | VTC2 | GDP-L-galactose phosphorylase; (1 of 1) 2.7.7.69 - GDP-L-galactose phosphorylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGCTTGGGTTGGCTTCAAGGTGTGGGG |
| Internal bar code: | TGCCCACGCGCACCTAGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 98.7254 |
| LEAP-Seq n confirming: | 852 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCGCAAACTCAACACA |
| Suggested primer 2: | TCTTTGCCTGCTGTTGATTG |