Insertion junction: LMJ.RY0402.147117_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147117
Chromosome:chromosome 4
Location:3774327
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCATCCCGTCCGTCTCCAAACTTCCAA
Internal bar code:CTTCTCGATAAGGCCTGAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:541
LEAP-Seq percent confirming:99.5161
LEAP-Seq n confirming:10489
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:TCTATGTCTCTGGGGCTGCT
Suggested primer 2:GCCTAGCTAGATGCGTGGAC