Insertion junction: LMJ.RY0402.147136_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147136
Chromosome:chromosome 10
Location:4466715
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g451600 SRR28,SRR28B (1 of 1) PF00059//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4); Lectin-domain glycosyl hydrolase intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGAGCCCCCCCCCCCCATGCGGCGGC
Internal bar code:TCACGCTGCTGGTCGCAGGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:875
LEAP-Seq percent confirming:98.5705
LEAP-Seq n confirming:2896
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGTCCCAGTGGAAGTTGA
Suggested primer 2:CCTCAGCGACATTACAGCAA