Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.147229 |
Chromosome: | chromosome_9 |
Location: | 558944 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g403800 | DYHB,ROC30,ROC54,ODA4 | Flagellar outer arm dynein heavy chain beta, involved in circadi | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTCGTACCTGCACGCGGAGGGAAAACGAA |
Internal bar code: | CGGTGTGGTATGGACAAAGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 957 |
LEAP-Seq percent confirming: | 93.9076 |
LEAP-Seq n confirming: | 447 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGAATTCGTCAAACTTGG |
Suggested primer 2: | CTACTTCCTGGACTCGCTGG |