| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147401 |
| Chromosome: | chromosome 16 |
| Location: | 3135786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665800 | SS4,SSS4 | Soluble starch synthase IV; (1 of 2) PTHR12526:SF304 - STARCH SYNTHASE 4, CHLOROPLASTIC/AMYLOPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGGCAGATGCTTGGATGTGTTGCGC |
| Internal bar code: | ATCGGTCCCCCATGGCCGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.6746 |
| LEAP-Seq n confirming: | 919 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTTGCTCGAGAGGGACA |
| Suggested primer 2: | CGCAGGCTGTCTAGGTAAGG |