| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147412 |
| Chromosome: | chromosome 12 |
| Location: | 1404948 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488100 | UPF3 | (1 of 1) K14328 - regulator of nonsense transcripts 3 (UPF3, RENT3); UPF3 regulator of nonsense transcripts, NMD protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCATACTCGTTCATAGCACTTCGGTGA |
| Internal bar code: | GTAAGGCCCCGGGGAGCGACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 58.0357 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGGAGTTTACGAAGCG |
| Suggested primer 2: | CGTATGCCGGTAGTGAAGGT |