| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147886 |
| Chromosome: | chromosome 10 |
| Location: | 2398968 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435400 | (1 of 1) PF03398 - Regulator of Vps4 activity in the MVB pathway (Ist1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGTTTAATACCGCGAAAGTGTAAGTCG |
| Internal bar code: | ATTCTGATCGTTAGGTGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 99.3553 |
| LEAP-Seq n confirming: | 1541 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGGGATTGATTTTGCTA |
| Suggested primer 2: | CCACTCACCTGGACCTGTTT |