| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148934 |
| Chromosome: | chromosome 12 |
| Location: | 695095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494750 | PRPS20 | Chloroplast ribosomal protein S20; (1 of 1) K02968 - small subunit ribosomal protein S20 (RP-S20, rpsT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGTTGCGTATGCGTTCTGCTCACGCT |
| Internal bar code: | TGAGTTACTATTCACATTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.6391 |
| LEAP-Seq n confirming: | 2485 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGCTGCGAAAGTGTATG |
| Suggested primer 2: | CGCGTCTATAACAAGTCGCA |