| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150070 |
| Chromosome: | chromosome 2 |
| Location: | 5555400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108750 | (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTATACAATACATTCATGTGAAGGAT |
| Internal bar code: | GACTCAGATTTAACCGTTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1175 |
| LEAP-Seq percent confirming: | 99.0452 |
| LEAP-Seq n confirming: | 2386 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACTCTTGGTCTGGGTG |
| Suggested primer 2: | AATGCGGATGTTACAGAGGG |