Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.150403 |
Chromosome: | chromosome_16 |
Location: | 2594157 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g661626 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACCCGCAAGGAGGTTGGAGCTGCGCAGA |
Internal bar code: | TTGGCGCAAGGTAAGGCTGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 946 |
LEAP-Seq percent confirming: | 99.3172 |
LEAP-Seq n confirming: | 1600 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATCGCTGAGCACTCTGCAC |
Suggested primer 2: | CTGCTCCTCGGTTTGATAGC |