| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151298 |
| Chromosome: | chromosome 10 |
| Location: | 2896574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440000 | RAA8,OPR120 | psaA mRNA trans-splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTTCCTCCACCCCCATCTCGCATGTGC |
| Internal bar code: | ATCACATGCCCGTGGCGTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTAGGACCGAGCATAC |
| Suggested primer 2: | GTGTCTGGACATGAAGGCG |