| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151324 |
| Chromosome: | chromosome 6 |
| Location: | 5133508 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281250 | CFA1 | Cyclopropane fatty acid synthase; (1 of 1) 2.1.1.79 - Cyclopropane-fatty-acyl-phospholipid synthase / Unsaturated-phospholipid methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGACATCTTCCAGCGCCCGCTGTGGCG |
| Internal bar code: | CCCCGGTTCGCACGTCTAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 99.8012 |
| LEAP-Seq n confirming: | 1004 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGGACACCATCTTTGC |
| Suggested primer 2: | CTGTGAATAAGGGAGCAGCC |