Insertion junction: LMJ.RY0402.151456_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151456
Chromosome:chromosome 16
Location:2601288
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g661650 (1 of 6) IPR001471//IPR016177 - AP2/ERF domain // DNA-binding domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCACGGAACAAGTGGGCTGCGCCCACGC
Internal bar code:GGATGGTCGATCATGACGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:223
LEAP-Seq percent confirming:70.1286
LEAP-Seq n confirming:709
LEAP-Seq n nonconfirming:302
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTGACGGGTGACGGTAG
Suggested primer 2:CTAACGTTTGGGAAGCGAAG