| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151495 |
| Chromosome: | chromosome 12 |
| Location: | 1285349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489150 | SHD1 | SH2-domain protein; (1 of 2) IPR000980 - SH2 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACAATGAGTGTACAATGGCCCTGTGG |
| Internal bar code: | TGACGGATTACCGGGCGTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 97.5028 |
| LEAP-Seq n confirming: | 3475 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCATGACTCGGCTAGGG |
| Suggested primer 2: | CACACAGACACACTGCAACG |