| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152088 |
| Chromosome: | chromosome 13 |
| Location: | 1945442 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576300 | GSC1,APH1 | (1 of 1) K06172 - anterior pharynx defective 1 (APH1); Gamma-secretase accessory subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGCTACCATCATTGAGCCATTCTGCCT |
| Internal bar code: | GTCGTCGTCCTACTTCGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.745 |
| LEAP-Seq n confirming: | 3520 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTTAGGCAGGATAGGG |
| Suggested primer 2: | CGATAGACTTCTTCGGCCTG |