| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152589 |
| Chromosome: | chromosome 4 |
| Location: | 3079387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225400 | (1 of 79) IPR011992 - EF-hand domain pair | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACCAAAACCTTGCGAGCGTAGTACGGG |
| Internal bar code: | GCAAGATATTTCGCCACGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 99.569 |
| LEAP-Seq n confirming: | 1155 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGGGGTGCTCAAAGATA |
| Suggested primer 2: | ATTGCTTGCTGCAACTGATG |