| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152612 |
| Chromosome: | chromosome 13 |
| Location: | 902712 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567750 | PRP18 | Pre-mRNA splicing factor; (1 of 1) K12817 - pre-mRNA-splicing factor 18 (PRPF18, PRP18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCTCTTTGCAGGCAAACGCAGGTGCAT |
| Internal bar code: | CAACCACGAGAGGTCAACAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.8141 |
| LEAP-Seq n confirming: | 537 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGTCCACTGACACCACT |
| Suggested primer 2: | ACCCAACACGAGCTCCATAC |