| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153316 |
| Chromosome: | chromosome 10 |
| Location: | 1408267 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428100 | TMG5 | (1 of 1) PTHR10631//PTHR10631:SF2 - N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE // N2, N2-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASES-LIKE PROTEIN; possible N2,N2-dimethylguanosine tRNA methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGGCGGCGGCAGTGACGAGGCCTCGGC |
| Internal bar code: | AACCCAGCACACAGCTGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 99.7508 |
| LEAP-Seq n confirming: | 1201 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGACCTGGCAAAGAAAG |
| Suggested primer 2: | TCCACCGATATACCTGAGCC |