| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153558 |
| Chromosome: | chromosome 2 |
| Location: | 2701092 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093500 | SSD1 | Sterol sensing 5-transmembrane protein; (1 of 1) PF03176 - MMPL family (MMPL) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGATGGCCGGTGGCGGGTGTTGAGGTG |
| Internal bar code: | GCCGCGCTATCGCTTTTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 98.6268 |
| LEAP-Seq n confirming: | 1221 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACATATCACCGCGAAAA |
| Suggested primer 2: | GTCACAGGCTTACCCACGTT |