| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153601 |
| Chromosome: | chromosome 10 |
| Location: | 2592371 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437150 | PPR6,CYCR5 | PentatricoPeptide Repeat protein 6; (1 of 2) K17710 - pentatricopeptide repeat domain-containing protein 1 (PTCD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCCGCCAGCCAGCAAGACCGACCGGAC |
| Internal bar code: | TAGTTTATCCCGCGCCTCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 99.6165 |
| LEAP-Seq n confirming: | 1039 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGGATAGTTAGGAGGC |
| Suggested primer 2: | ACGAGTAGCTGCCAAAGGAA |