Insertion junction: LMJ.RY0402.153731_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153731
Chromosome:chromosome 1
Location:7339274
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g052750 (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATCTGCATGTGCCCGTACGACAAGTAC
Internal bar code:GGGTCGATGAAGGGTAGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:809
LEAP-Seq percent confirming:99.7831
LEAP-Seq n confirming:460
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTTTAGACCACTCCCCA
Suggested primer 2:GCTCAAGTGAGTGAGGGAGG