Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.153763 |
Chromosome: | chromosome 6 |
Location: | 1104644 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g256700 | (1 of 1) IPR014001//IPR026127//IPR027417 - Helicase superfamily 1/2, ATP-binding domain // Probable RNA helicase SDE3 // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCCTCGAGCAAGCCCTGCAGCTACGCC |
Internal bar code: | TCGACACGTCCATAGCGCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 681 |
LEAP-Seq percent confirming: | 75.3919 |
LEAP-Seq n confirming: | 481 |
LEAP-Seq n nonconfirming: | 157 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTGTGGATTGTGTTGGTG |
Suggested primer 2: | GCCAAATGTGTCCATCAGTG |