Insertion junction: LMJ.RY0402.153986_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153986
Chromosome:chromosome 13
Location:3276980
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g586050 (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCAAACCCCTGCCACGTGCCCCGCGCCC
Internal bar code:GCTCAAGCACCGGACGGATACC

Confirmation - LEAP-Seq

LEAP-Seq distance:456
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCATTTTCCTTGGACC
Suggested primer 2:ACTGCGATTGGATGAAGGAC