Insertion junction: LMJ.RY0402.154368_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154368
Chromosome:chromosome 5
Location:1227753
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g245351 (1 of 1) PF00059//PF00530//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACCTGTCTGCGGGCTGGAACAACGAT
Internal bar code:CACTCGGGATCGACGAGCAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:980
LEAP-Seq percent confirming:99.9184
LEAP-Seq n confirming:1225
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCCTGCCAGTACGTTCC
Suggested primer 2:AGCACACACACTCACCGAAG