| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154417 |
| Chromosome: | chromosome 6 |
| Location: | 1574275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260450 | LCI20 | Putative 2-oxoglutarate/malate translocator; (1 of 1) K03319 - divalent anion:Na+ symporter, DASS family (TC.DASS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGTTTATGTGTCCACAAACCGCCGATC |
| Internal bar code: | TAATAGGAAGATTGGGTTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 99.7647 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCAATTTCCATGGACGC |
| Suggested primer 2: | CTACGGCTCTGGCTTTATGC |