| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154533 |
| Chromosome: | chromosome 11 |
| Location: | 1943446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468356 | TPR7,TPR | (1 of 1) K09291 - nucleoprotein TPR (TPR, MLP1, MLP2); Tetratricopeptide-repeat protein 7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAAGTGTCTCGCAACGGCCTGCCAAGC |
| Internal bar code: | CCCTTAAATCAGTGGCACAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.8577 |
| LEAP-Seq n confirming: | 2806 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCGGACTGAGGTGCTT |
| Suggested primer 2: | TGCTAGCGGTTTCTCCCTAA |