| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154691 |
| Chromosome: | chromosome 12 |
| Location: | 7539687 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555700 | DNJ15 | DnaJ-like protein; (1 of 5) IPR000104//IPR001623 - Antifreeze protein, type I // DnaJ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGTATAGTCGATGTGAGCTGAGCGTTG |
| Internal bar code: | GGTATGGGCGCGCAGTGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 99.5749 |
| LEAP-Seq n confirming: | 14289 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAAGCAGGGCCTGACAAT |
| Suggested primer 2: | AGCAAACACAGCACGTATGC |